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KMID : 0364819880260010013
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Korean Journal of Microbiology
1988 Volume.26 No. 1 p.13 ~ p.19
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Characterization of R plasmid pKU41 from Pseudomonas putida KU190
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Lee, Yoon-Hee/이윤희
Joo, Mee-Ja/Lee, Yung-Nok/주미자/이영록
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Abstract
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1
The location of R-determinants, Ap´ and Tc´, and replication origin in pKU41 determined using the construction of miniplasmid by the BamHI and the Hindlll restriction fragment from pKU41 and the cloning of the restriction fragments from pKU41 into pSY343. The gene encoding resistance to ampicillin (Ap) as well as replication origin in pKU41 were located on the region overlapping BamHI B fragment and Hindlll A fragment. The gene encoding resistance to tetracycline (TO was located on the region of the HindHI C fragment, which was cleaved by BamHI as well.
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